y-Subunit of Cyclic Guanosine Monophosphate Phosphodiesterase in Rats

نویسندگان

  • Jianming Ren
  • Akio Yamazaki
  • Hitoshi Shichi
چکیده

Methods. Bovine Py was expressed in Escherichia coli cells and purified by fast protein liquid chromatography. Lewis rats were immunized by a single footpad injection of Py emulsified in complete Freund's adjuvant. Clinical and histopathologic changes in the eye and pineal gland were examined. Lymphocytes were prepared from the lymph nodes of rats with uveitis and transferred by intraperitoneal injection to naive recipient rats.

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تاریخ انتشار 2005